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Leica EM PACT2/RTS 高压冷冻仪
相对于化学固定,冷冻固定在毫秒级时间内完成固定过程,时间分辨率无损失,能够捕捉细胞生理活动的瞬间,最大限度的保存蛋白质、酶、抗原的原始结构和活性,保持膜系统原有的渗透性,且能够固定可溶性小分子和离子。
高压冷冻固定是最好的冷冻固定方法。在约2100个大气压下,水的冰点下降至约-20°C,并且冰晶形成和生长速度大大降低,从而实现快速冷冻固定而不形成冰晶,有效固定深度可达200微米。
Leica EM PACT2 专为分子和细胞生物学家设计,满足细胞“活体”结构和功能研究的需求。利用快速传输系统Leica EM RTS还可以进行光镜/电镜关联实验,只需不到5秒钟,即可对光镜观察活体样品进行固定。