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Leica EM HPM100 高压冷冻仪
相对于化学固定,冷冻固定在毫秒级时间内完成固定过程,时间分辨率无损失,能够捕捉细胞生理活动的瞬间,最大限度的保存蛋白质、酶、抗原的原始结构和活性,保持膜系统原有的渗透性,且能够固定可溶性小分子和离子。
高压冷冻固定是最好的冷冻固定方法。在约2100个大气压下,水的冰点下降至约-20°C,并且冰晶形成和生长速度大大降低,从而实现快速冷冻固定而不形成冰晶,有效固定深度可达200微米。LeicaEM HPM100 能够对超大样品进行高压冷冻固定(最大Ø5mm×0.6mm厚),多种样品夹系统可供选择,可以满足任何应用领域。